Instruments

DFCI Flow Cytometry’s facilities serve researchers in DFCI laboratories, external labs, and corporate settings. Interested researchers are invited to visit the facilities and meet with staff to discuss research projects that would be enhanced by employing flow cytometry technology.

 

 

Jimmy Fund Facility
(JF 415)

Sorters

  • BD FACSAria IIu
    • 3 Lasers 9 Parameters
    • Lenti Virus & Human Primary sample sorting not allowed
      NO SORTING OF KNOWN PATHOGENS
  • BD FACSAria II SORP UV
    • BioPROtect III Hood
    • 6 Lasers 17 Parameters
    • Lenti Virus & Human Primary sample sorting allowed
      NO SORTING OF KNOWN PATHOGENS
    • See Lenti Virus policies
Sorter specifications

Analyzers

  • BD LSR II (Lenti Virus analysis)
    • 3 Lasers 12 Parameters
    • Lenti Virus analysis must be performed on the LSR using the HTS plate reader
    • See Lenti Virus policies
  • BD LSRFortessa
    • 4 Lasers 10 Parameters
    • Lenti Virus & Human Primary analysis not allowed
Analyzer specifications Rates and scheduler outside of the Dana-Farber Jimmy Fund building

Hematologic Neoplasia Facility (Mayer 584)

Sorters

  • BD FACSAria II SORP
    • 4 Lasers 16 Parameters
    • Lenti Virus & Human Primary sample sorting allowed
      KNOWN PATHOGEN SORTING NOT ALLOWED
  • BD FACSAria II SORP UV
    • 5 Lasers 18 Parameters
    • Lenti Virus & Human Primary sample sorting allowed
      KNOWN PATHOGEN SORTING NOT ALLOWED
Sorter specifications

Analyzers

  • BD LSRFortessa (3)
    • 4 Lasers 17 Parameters
    • Lenti Virus & Human Primary sample analysis allowed using HTS plate reader only
  • BD FACSCanto II HTS (2)
    • 3 Lasers 8 Parameters
    • Lenti Virus  & Human Primary sample analysis allowed using HTS plate reader only
  • BD FACSCanto II Carousel
    • 3 Lasers 8 Parameters
    • Lenti Virus & Human Primary analysis not allowed
Analyzer specifications Rates and scheduler outside of the Dana-Farber Mayer building