Lentivirus & Human Primary Cells (unfixed) policies and laboratory practices

  • Sorting of Unfixed Human Primary or Lentivirus-infected cells
    KNOWN PATHOGEN SAMPLES CANNOT BE SORTED IN THIS FACILITY

Current revision date: 07/01/2015

Description of possible risks

Lentiviruses can deliver a significant amount of viral RNA into the DNA of the host cell and have the unique ability among retroviruses of being able to infect non-dividing cells, so they are one of the most efficient methods of a gene delivery vector. HIV, SIV, and FIV are all examples of lentiviruses.

Lentivirus Sorting practice

Cells infected with lentiviral particles will be sorted on the designated BD FACSAria II SORP sorters housed within the Baker containment hoods only.
Lentivirus Standard Operating Procedure (07-2015)   Jimmy Fund Core
Lentivirus Standart Operating Procedure (07-2015)   HemNeo Core


Human Primary Cells (HPC)Sorting practice

Human Primary Cells will be sorted on the designated BD FACSAria II SORP sorters housed within the Baker containment hoods only.

HPC Standard Operating Procedure Sorting (07-2015) Jimmy Fund Core
HPC Standard Operating Procedure Sorting  (07-2015) HemNeo Core


Post-sorting procedure

After cell sorting is complete the operator is responsible for disinfecting the areas used for lentiviral sorting. The following areas must be disinfected with AMPHYL and the Super Sani cloth wipes to clean accordingly.

  • Sort chamber
  • Sample holder
  • Immediate surrounding surfaces
  • Glass doors
  • Computer workstation area and key board

The researcher is responsible for removal and disposal of all gloves, disposable lab coats, and all wipes used to disinfect the sorter and affected areas.

Contact the Flow Cytometry Facility (617-632-3179) or Suzan Lazo (617-632-4571) for more information.

Analysis of lentivirus-infected cells

Current revision date: 07/01/2015

Description of possible risks

Lentiviruses can deliver a significant amount of viral RNA into the DNA of the host cell and have the unique ability among retroviruses of being able to infect non-dividing cells, so they are one of the most efficient methods of a gene delivery vector. HIV, SIV, and FIV are all examples of lentiviruses.

Lentivirus Standard Operating Procedure Analysis   Jimmy Fund Core

Lentivirus Standard Operating Procedure Analysis   HemNeo Core


 Analysis of Human Primary Cells  HPC(unfixed)

HPC Standard Operating Procedure Analysis              Jimmy Fund Core
HPC Standard Operating Procedure Analysis              HemNeo Core

The researcher is responsible for removal and disposal of gloves, disposable lab coat, and all wipes used to disinfect the analyzer and affected areas.

Contact the Flow Cytometry Facility (617-632-3179) or Suzan Lazo (617-632-4571)